Accurate quantitation of antibiotic penetration through staphylococcal biofilms

Bin, L., McGiffin, D., Nguyen, T., Wang, L., Sun, Y., Ye, L., Han, M., Sheng, C., Lee, T., Aguilar, M., Peleg, A., Qu, Y. BioFilm DOI: 10.1016/j.bioflm.2025.100316

Abstract: Limited antimicrobial penetration is an important mechanism underlying antimicrobial resistance of biofilms and has often been incorrectly quantitated. We adopted a rationalized antibiotic agar-diffusion model to accurately interpret experimental results of a widely-accepted biofilm penetration.